Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Bactericidal activity of 50% SPPP and 50% HISPPP decomplemented by heating at 56 °C for 30 min against wild-type (WT) Salmonella enterica subsp. enterica strains.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Survival of Salmonella enterica strains during eight passages in 50% human serum from platelet-poor plasma (SPPP). Black/bottom line–survival of Salmonella enterica in 50% SPPP counted as log 10 of colony forming units per ml (CFU/mL −1 ) connected with dashed trendline formula; blue/upper line (control)–survival of bacteria in heat-inactivated (56 °C, 30 min) sera (HISPPP); SS–serum sensitive; SR–serum resistant; ( A )— S . Isaszeg P8 (SR); ( B )— S . Erlangen P8 (SR); ( C )— S . Hammonia P8 (SR); ( D )— S . Enteritidis ATCC 13076 P8 (SR); ( E )— S . Typhimurium ATCC 14028 P8 (SS); ( F )— S . Senftenberg P8 (SS).

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: Clinical Proteomics, Control, Bacteria

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: SDS-PAGE analysis of the membrane protein (MP) profiles (12 μg/10 μL per well) of the studied Salmonella enterica isolates. Definitions of abbreviations: M—protein marker (AppliChem, Darmstadt, Germany), Line 1— S . Isaszeg, Line 2— S . Erlangen, Line 3— S . Hammonia, Line 4— S . Typhimurium ATCC 14028, Line 5— S . Enteritidis ATCC 13076, Line 6— S . Senftenberg; ( A ) WT (serum sensitive, SS) Salmonella strains, ( B ) Salmonella strains passaged eight times in 50% SPPP (P8); SR—serum resistant. The bottom half of represents densitograms obtained with Image Lab. V. 4.1 (Bio-Rad, Hercules, CA, USA).

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: SDS Page, Membrane, Marker

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Dot blot C1q and C3c-binding assays performed for membrane proteins (MPs) isolated from serum-resistant (SR) and serum-sensitive (SS) strains. 1—MPs of SS S . Hammonia, 2—MPs of SR S . Hammonia P8, 3—MPs of SS S . Typhimurium ATCC 14028, 4—MPs of SS S . Typhimurium ATCC 14028 P8. MPs in concentrations of ( A ) (25 × diluted in H 2 O miliQ , 0.24 mg/mL −1 ) and ( B ) (50 × diluted in H 2 O miliQ, 0.12 mg/mL −1 ) were loaded onto nitrocellulose membrane. K1—positive control 1; K2—positive control 2; K3—negative control 1, instead of MPs PBS was used; K4, negative control 2—instead of SPPP PBS was used.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: Dot Blot, Binding Assay, Membrane, Isolation, Positive Control, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Minimal inhibitory concentrations for six antibiotics (MIC, mg L −1 ) and three disinfectants (MIC, %) in Salmonella enterica passages in 50% SPPP.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Virulence genes (VGs) profiles of Salmonella enterica strains.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Biofilm production by wild-type Salmonella enterica strains and passaged in 50% SPPP determined by microtiter plate assay at 28 °C and 37 °C. Interpretation of the results: OD ≤ 0.011–non-biofilm producer; 0.011 < OD ≤ 0.022—weak-biofilm producer; 0.022 < OD ≤ 0.044—moderate-biofilm producer; 0.044 < OD—strong-biofilm producer; SS—serum sensitive, SR—serum resistant.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Changes of cells’ hydrophobicity after passages in 50% SPPP.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Morphology of bacterial colonies produced by serum-sensitive (SS) and serum-resistant (SR) strains. ( A ) Salmonella strains not passaged in SPPP, ( B ) Salmonella strains passaged eight times in 50% SPPP; 1— S . Erlangen, 2 — S . Hammonia, 3 — S . Typhimurium ATCC 14028.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: Produced

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Swarming motility assay. Definitions of abbreviations: ( A )— Salmonella strains not passaged in SPPP, ( B )— Salmonella strains passaged eight times in 50% SPPP; 1— S . Erlangen, 2— S . Hammonia, 3— S . Isaszeg, 4— S . Senftenberg, 5— S . Enteritidis ATCC 13076, 6— S . Typhimurium ATCC 14028.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: Motility Assay

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: Swimming motility assay. Definitions of abbreviations: ( A )—the average (n = 3) diameter of bacterial cultures (WT and P8) grown on 0.3% soft agar supplemented with TTC at 28 °C and 37 °C. ( B )—differences between inoculum growth of pairs S . Erlangen (SS)/ S . Erlangen P8 (SR) and S . Typhimurium ATCC 14028 (SS)/ S . Typhimurium ATCC 14028 P8 (SS); WT—wild-type strain (SS), P8—strain passaged eight times in 50% SPPP; * indicates a statistically significant difference in values ( p < 0.05) with respect to the WT strains, SS—serum sensitive, SR—serum resistant.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: Motility Assay

Journal: International Journal of Molecular Sciences

Article Title: The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

doi: 10.3390/ijms24010883

Figure Lengend Snippet: The origin and antigenic characteristics of Salmonella enterica strains used in the study. Definitions of abbreviations: P8—strain passaged eight times in SPPP at 37 °C; PCM—Polish Collection of Microorganisms, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland; IP—Institute Pasteur, Paris, France; NBIMCC—National Bank of Industrial Microorganisms and Cell Cultures, Sofia, Bulgaria; ATCC—American Type Culture Collection; * sensitive to 75% normal human serum (NHS) at 37 °C obtained from five volunteers who had not received any antimicrobial drug treatment for six months [ 10 ]; ** sensitive or resistant to 50% human serum from platelet-poor plasma (SPPP), sterile-filtered, mycoplasma and virus tested (Sigma-Aldrich, Poznan, Poland) at 37 °C (this study); *** resistant to 50% normal human serum (NHS) at 37 °C obtained from the Regional Center of Transfusion Medicine and Blood Bank, Wroclaw, Poland (120 donors) and approved by the authors’ institutional review board [ 16 ]; NeuAc/Kdo—the ratio of N -acetylneuraminic acid (NeuAc) to 3-deoxy-D-manno-2-octulosonic acid (Kdo) analyzed with GLC-MS; NT—not tested.

Article Snippet: enterica , Typhimurium , 1 ,4,[5],12 , i,1,2 , S . Typhimurium P8 , ATCC 14028 , Sensitive **, this study , NT.

Techniques: Surround Optical-fiber Immunoassay, Clinical Proteomics, Sterility, Virus